## How to calculate Vmax and Km from a Lineweaver and Burk plot

A quick guide for my students on how to use excel to get Km and Vmax.

From DrDavidSmith

A quick guide for my students on how to use excel to get Km and Vmax.

From DrDavidSmith

Please watch: "General structure of an amino acid (PK)" https://www.youtube.com/watch?v=LXltM6WYmQQ -~-~~-~~~-~~-~-

From eBiochem// eBioStat

The Michaelis constant is the substrate concentration at which an enzyme operates at one half of its maximum velocity. How can you calculate this constant ...

A good way of finding the Michaelis constant and the (half) maximum velocity of a certain enzyme reaction is to work with a linearized version of your data. Excel ...

In this video I have explained how to calculate the value of Km and Vmax for an enzyme substarte reaction using Michaelis-Menten equation. Thanks to Amanda ...

From Dr.Mungli

You need to know the 3 parts of the graph: a) initially linear since many free enzymes available at the lower concentration of substrate. b) rate begins to slow as ...

From Richard Thornley

Using Excel to do the Lineweaver-Burk plot.

From Mark Temple

A specific case for B.anthracis where we calculate by Excel 2 of the most important parameters needed for Growth kinetics.

From ElIsrolak

Compétences connexes : - Réaction enzymatique - Savoir fixer les ligne et les colonnes avec des $ - avoir déterminé préalablement les vitesses initiale de ...

Discussion of the meaning and graphical determination of the kinetic constants of Km & Vmax.

From Casey M

Check out our physics videos at www.physicseh.com This shows you how to calculate velocity, acceleration and kinetic energy using Excel.

From PhysicsEH

From Michael Parkinson

From Casey M

How to calculate reaction rate from your absorbance data!

From kirk kawagoe

using a Lineweaver Burk plot to analyse enzyme data This work is licenced under the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported ...

From Peter Klappa

In this screencast, we will look at using two methods to estimate parameters: fitting a manipulated equation to a line, and non-linear least squares. The example ...

From Gregory Reeves

Moof's Medical Biochemistry Video Course: http://moof-university.thinkific.com/courses/medical-biochemistry-for-usmle-step-1-exam For Related Practice ...

From Moof University

Moof's Medical Biochemistry Video Course: http://moof-university.thinkific.com/courses/medical-biochemistry-for-usmle-step-1-exam For Related Practice ...

From Moof University

Lineweaver-Burk Plot: https://en.wikipedia.org/wiki/Lineweaver%E2%80%93Burk_plot#/media/File:Lineweaver-Burke_plot.svg.

From Anna G

How to use excel to create a direct linear plot. Need to use (n^2 -n) / 2 to find the median value.

From Kim O

This shows SCH4U students how to use Excel to plot conc vs time 1/conc vs time ln conc vs time and instantaneous rate change vs time (first differences, kinda)

From Jon McGoey

For Dr. Lefler's BCH 367 course at ASU Tempe campus. Enzyme Kinetics lab tutorial (Part 1). Link to Part 2: https://youtu.be/0uYHnjjFcHw.

From ryanashi

From Catalyst University

From Catalyst University

Los datos cinéticos para la reacción catalizada por la prostaglandina endoperóxido sintasa se muestran a continuación. a)Determine la Vmax y la Km de la ...

From WissenSync

This video will show you how to calculate the rate of the reaction in the Enzyme Activity lab.

From Chris Romero

Created by Ross Firestone. Watch the next lesson: ...

From khanacademymedicine

se describe el procedimiento para obtener los valores de Velocidades iniciales (V0) a partir de las absorbancias medidas en los tubos de reacción. Ademas se ...

From mrcreyes

using an Eadie Hofstee plot to analyse enzyme data This work is licenced under the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported ...

From Peter Klappa

From Chemical Mack

In this video I have explained how to calculate Km and Vmax of an enzyme in Lineweaver Burk double reciprocal plot. The Lineweaver–Burk plot was widely ...

From Dr.Mungli

Lab 7 Excel video: Enzyme Activity graph (c) Veniamin "Ben" Slavitskiy, Emmanuel College, 2016.

From venka23

How to add error bars to a LWB graph.

From oldsynner

For Dr. Lefler's BCH 367 course at ASU Tempe campus. Enzyme Kinetics lab tutorial (Part 2). Link to Part 1: https://www.youtube.com/watch?v=6RMEokEDBaw.

From ryanashi

Donate here: http://www.aklectures.com/donate.php Website video link: http://www.aklectures.com/lecture/lineweaver-burk-plot-and-reversible-inhibition ...

From AK LECTURES

This enzyme catalysis lecture explains about Michaelis menten plot and equation. http://shomusbiology.com/ Download the study materials here- ...

From Shomu's Biology

To get a better overview of differences between groups or samples, you may want to plot the median for each group or sample with a minimum, maximum, 25% ...

In this video we export our data from Graphical to Microsoft Excel.

From Eric D. Brass Sr.

This lecture explains about double reciprocal plot of enzyme regulation. http://shomusbiology.com/ Download the study materials here- ...

From Shomu's Biology

Using excel and solver to find vmax km kcat and ki.

From Raul Orozco

Determining the dissociation constant Ki for a competitive inhibitor.

From Peter Klappa

For more Biochemistry videos: http://freebiologyschool.blogspot.com/p/biochemistry.html Derive a linear plot from the Michaelis Menton-Equatoin.

From Mike Birkhead

In this video I'm explaining about Km and Vmax using Micahelis-Menten plot and Linweaver-Burk plot. This is the continuation of enzyme kinetics videos (part ...

From Dr.Mungli

From king lord

Excel 2003 graphs.

From lhartscience

Applications and skills: Determination of the maximum rate of reaction (Vmax) and the value of the Michaelis constant (Km) for an enzyme by graphical means, ...

From Mike Sugiyama Jones

Compétences annexes : - Savoir réaliser un graphique sur excel - Savoir compléter les axes par rappel automatique des données - Savoir ce qu'est une ...